PTEN Knockout cell line (PANC-1)

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location： Home > Products > KO Cell Lines > PTEN Knockout cell line (PANC-1)

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PTEN Knockout cell line (PANC-1) Order

PTEN Knockout cell line (PANC-1)
Catalog#
YKO-HJ117
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTEN Knockout cell line (PANC-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PTEN Knockout cell line (PANC-1)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Human
Gene Name
PTEN
Gene ID
5728
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_E1AK(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript PTEN-201)
Validation: PCR+Sanger Sequencing
PTEN Knockout cell line (PANC-1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:PANC-1		 STR Info (Cell bank)
Cell Line:PANC-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 11111111
D7S820 810810
D16S539 11121111
VWA 15151515
TH01 7878
AMEL XXXX
TPOX 811811
CSF1PO 10121012
D12S391 2222
FGA 2121
D2S1338 2324
D21S11 2828
D18S51 1212
D8S1179 1415
D3S1358 1717
D6S1043 1112
PENTAE 714
D19S433 1116
PENTAD 1414
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTEN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PANC-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTEN gene knockout PTEN Knockout cell line (PANC-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PTEN Knockout cell line (PANC-1)
PTEN Knockout cell line (PANC-1)
Catalog#
YKO-HJ117
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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